Obul Reddy, B. and Giridhar, P. and Ravishankar, G. A. (2001) In vitro rooting of Decalepis hamiltonii Wight & Arn., an endangered shrub, by auxins and root-promoting agents. Current Science, 81 (11). pp. 1479-1482.
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Abstract
In the present study micropropagation of an endangered
shrub, Decalepis hamiltonii Wight & Arn.,
optimization and standardization of efficient methods
for rooting of in vitro-derived micro-shoots by using
phloroglucinol (PG), activated charcoal and CoCl2
have been achieved. Transfer of in vitro-derived shoots
to MS medium supplemented with 8.8 mM indole-3-
butyric acid (IBA) and 1.43 mM indole-3-acetic acid
resulted in root induction. In another treatment, IBA
was found to be a good root-promoting agent, wherein
dipping of explants in 4.4 μM IBA for 30 min and
subsequent inoculation on MS basal medium was also
beneficial for root induction. Successful field transfer
was accomplished in rooted plantlets.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Decalepis hamiltonii, culinary spice, aromatic roots, micropropagation protocols |
| Subjects: | 500 Natural Sciences and Mathematics > 10 Plants > 06 Trees And Shrubs 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture |
| Divisions: | Plant Cell Biotechnology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 05 Jan 2009 09:43 |
| Last Modified: | 28 Dec 2011 09:38 |
| URI: | http://ir.cftri.res.in/id/eprint/2118 |
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