MIG1 overexpression causes flocculation in Saccharomyces cerevisiae.

Shankar, C. S. and Ramakrishnan, M. S. and Umesh Kumar, S. (1996) MIG1 overexpression causes flocculation in Saccharomyces cerevisiae. Microbiology, 142 (. pp. 2663-7. ISSN 1350-0872

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Abstract

MIG1, encoding a C2H2 zinc-finger repressor protein involved in carbon catabolite repression, was found to play a role in non-sexual flocculation of Saccharomyces cerevisiae. Disruption of MIG1 in a flocculent mutant strain of NCYC 227, resulted in a non-flocculent phenotype. Expression of MIG1 on a 2 mu pRS426 vector in a non-flocculent strain, YM 4134, caused flocculation; MIG1 on a high-copy-number LEU2-d plasmid caused intense flocculation in the same strain. Mutations in the SSN6 and TUP1 genes confer a flocculent phenotype in non-flocculent strains of S. cerevisiae, and it has been shown that Mig1 can tether the Ssn6p-Tup1p complex to the regulatory regions of glucose-repressible genes. Mutations in tup1 in a MIG1 background caused flocculation while double mutants of TUP1 and MIG1 did not flocculate. Based on these results, a model for the role of MIG1 in flocculation gene regulation is proposed.

Item Type:	Article
Uncontrolled Keywords:	flocculation, regulation, MIG 1, SSN6-TUP1
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 19 Yeast 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	22 Mar 2018 05:42
Last Modified:	22 Mar 2018 05:42
URI:	http://ir.cftri.res.in/id/eprint/2232

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