Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with sodium chloride solutions.

Sreeramulu, G. and Singh, N. K. (1995) Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with sodium chloride solutions. Electrophoresis, 16 (3). pp. 362-5. ISSN 0173-0835

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Abstract

A novel method for destaining of polyacrylamide gels, stained with Coomassie Brilliant Blue R-250, is described, based on the use of 0.5 M NaCl in water as the destainer, requiring only 2-3 h. Concentrated (> 2 M) or dilute (< 0.1 M) salt solutions were unsuitable. The method affords the advantage that expensive organic solvents, such as methanol, acetic acid or trichloroacetic acid, are not needed. Furthermore, salt destaining results in darker purple-blue protein bands as compared to the pale blue color with e.g. methanol/acetic acid destaining.

Item Type:	Article
Uncontrolled Keywords:	Destaining Sodium dodecyl sulfate-polyacrylamide gel electrophoresis Wheat Prolamins
Subjects:	600 Technology > 08 Food technology > 14 Physical properties > 01 Colours 600 Technology > 08 Food technology > 21 Cereals > 04 Wheat
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	21 Mar 2018 10:28
Last Modified:	21 Mar 2018 10:28
URI:	http://ir.cftri.res.in/id/eprint/2256

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