Development of Enzyme-Linked Immunosorbent Assay for Evaluation of Chapati-Making Quality of Wheat Varieties

Prabhasankar, P. and Sai Manohar, R. (2002) Development of Enzyme-Linked Immunosorbent Assay for Evaluation of Chapati-Making Quality of Wheat Varieties. Journal of Agricultural and Food Chemistry, 50 (25). pp. 7455-7460.

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Abstract

<p align="justify">An indirect enzyme-linked immunosorbent assay (ELISA) has been developed for evaluation of chapati-making quality of wheat varieties. Polyclonal antibodies against gliadin, low molecular weight glutenin (LMG), and high molecular weight glutenin (HMG) were developed and utilized in the ELISA. Twenty-eight Indian wheat varieties were utilized in the ELISA. Out of these antibodies, an antigliadin antibody response was negatively correlated with farinograph water absorption ( r) -0.89 at P less than 0.01), chapati dough water absorption ( r) -0.91 at P less than 0.01), overall chapati sensory score ( r)-0.95 at P less than 0.01), chapati puffed height score ( r) -0.95 at P less than 0.01), and positively correlated with shear value of chapati ( r) 0.76 at P less than 0.01). Anti-LMG antibody response was not correlated with any of these parameters, whereas anti-HMG response positively correlated with chapati dough water absorption ( r) 0.44 at P less than 0.05), farinograph water absorption ( r) 0.45 at P less than 0.05), and overall chapati sensory score ( r) 0.44 at P less than 0.05), and negatively correlated with shear value ( r)-0.38 at P less than 0.05) and chapati puffed height ( r) -0.44 at P less than 0.05). The results indicate that wheat varieties with good chapati-making quality were having less antigliadin antibody response.</p>

Item Type:	Article
Uncontrolled Keywords:	Chapati wheat ELISA rheology pasting characteristics
Subjects:	600 Technology > 08 Food technology > 14 Physical properties 600 Technology > 08 Food technology > 21 Cereals > 04 Wheat
Divisions:	Flour Milling Bakery and Confectionary Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	12 Dec 2005
Last Modified:	28 Dec 2011 09:25
URI:	http://ir.cftri.res.in/id/eprint/253

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