Anthocyanin production in callus cultures of Daucus carota as influenced by nutrient stress and osmoticum.

Daucus carota callus developed red pigments under the
influence of indole-3 acetic acid and kinetin. Maximum yield
of anthocyanin at the end of 3 weeks was 5.4% on dry weight
basis. The callus subjected to phosphate and nitrate stress
produced 7.2% and 8.5% anthocyanin respectively. Feeding of
sucrose at 7.5% level resulted in production of 15%
anthocyanin. Mannitol as osmoticum had positive influence on
anthocyanin production.