Characterization of cell wall components from the endosperm of sorghum varieties varying in hardness.

Kavitha, R. and Chandrashekar, A. (1993) Characterization of cell wall components from the endosperm of sorghum varieties varying in hardness. Carbohydrate Polymers, 22 (2). 107-115, 22 ref..

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Abstract

Cell walls, isolated from 2 var. of sorghum [M-35-1 (soft) and E-35-1 (hard)] endosperm varying in hardness, were extracted with 1, 4 or 8M alkali and subsequently neutralized to give an insoluble 'A' and a soluble 'B' fraction. The soluble 'B' fractions had higher arabinose-to-xylose and pentose-to-hexose ratios compared with their respective 'A' fractions. The residue after extraction with 8M alkali in the soft var. contained 16% pentoses, whereas the residue from the hard var. had only traces of pentoses. On further fractionation of 1M 'B' on DEAE cellulose, a glucose-rich unbound fraction and a bound fraction containing a considerable amount of pentoses were obtained. Prolamins (sorghum storage proteins) were associated with isolated cell wall fractions. Antibodies were raised against 3 of the cell wall polymer fractions and were capable of recognizing specific and common epitopes. Results indicate that the more loosely held polymers are rich in glucose, while the tightly held polymers, probably attached to cellulose, are made up of pentoses.

Item Type:	Article
Uncontrolled Keywords:	CARBOHYDRATES-; CELLS-; CEREALS-; SORGHUM-; SUGARS-; CELL-WALLS
Subjects:	600 Technology > 08 Food technology > 21 Cereals > 06 Sorghum 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry
Divisions:	Grain Science and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	02 Jun 2011 11:17
Last Modified:	28 Dec 2011 09:44
URI:	http://ir.cftri.res.in/id/eprint/3374

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