Lipid profile of cultured cells of apple (Malus sylvestris) and apple tissue.

The potentiality of apple cell cultures to synthesize not only higher quantities of
lipids than apple fruit but also different classes of lipids is noted. Total lipid was 15-fold
higher in apple callus than in the original tissue. On callusing, linoleic acid decreased from
66% to 14%, while linolenic acid showed a very large increase from 0·9% to 44%. Stearic and
oleic acids also increased in callus. The relative amounts of sterol/hydrocarbon and diglyceride
fractions were higher in callus cultures, while apple tissue showed higher levels of triglycerides
and sterol. Phosphatidylethanolamine and phosphatidylglycerol seemed to be
newly synthesized during callusing while other phospholipids such as lysophosphatidylcholine,
lysophosphatidylethanolamine, phosphatidylinositol and phosphatidic acid
decreased. There was much higher glycolipid in apple callus than in the original tissue. The
ratio of neutral lipid to polar lipid was higher in apple than in apple callus.