Molecular mechanism of action of butyric acid in Ehrlich ascites tumor cells.

Salimath, P. V. and Tabassum, A. and Anupama, E. G. and Bindu, Malini. and Preeti, G. B. and Salimath, P. V. (1999) Molecular mechanism of action of butyric acid in Ehrlich ascites tumor cells. Nutrition Research, 19 (4). pp. 589-600.

PDF Nutrition Research, Volume 19, Issue 4, April 1999, Pages 589-600.pdf - Published Version Restricted to Registered users only Download (855kB)

Abstract

We have shown that butyric acid (in viva) decreased cell proliferation of Ehrlich Ascites Tumor (EAT) cells. This effect of butyric acid is attributed to induction of apoptosis in EAT cells, as evidenced by prevalent apoptotic morphology in EAT cells. Molecular effects of butyric acid (in vivo and -i-n situ) included a dose dependent inhibition of generation of Reactive Oxygen Species(R0.S) with IC50 value of 5 uM. Butyric acid inhibited neovascularization in the peritoneum of EAT bearing mice. The absence of permeability factor(s) in exudate of mice bearing EAT cells treated with butyric acid, can either be due to inhibition of secretion of growth factors or repression of their expression by butyric acid. Further, butyric acid not only activated a cytosolic phosphatase but also induced its expression as evidenced by immunological staining, using anti-phosphatase antibodies.

Item Type:	Article
Uncontrolled Keywords:	Butyric Acid, EAT Cells, Apoptosis, ROS generation, Angiogenesis, Phosphatase.
Subjects:	600 Technology > 08 Food technology > 11 Food Biochemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	20 Feb 2012 05:07
Last Modified:	20 Feb 2012 05:07
URI:	http://ir.cftri.res.in/id/eprint/4667

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