Properties of mitochondria isolated from the cotyledons of (Phaseolus vulgaris).

Venugopal, T. D. and Patwardhan, M. V. (1973) Properties of mitochondria isolated from the cotyledons of (Phaseolus vulgaris). Indian Journal of Biochemistry and Biophysics, 10. pp. 109-112.

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Abstract

Actively respiring mitochondrial fraction has been Isolated from the cotyledons of Phaseolus vulgaris. Polarographic studies indicate a respiratory control ratio of 5,0 and ADP/O ratio of I'Z with succinate as substrate. Michaelis Constant values for ADP, succinate and inorganic phosphate are 76, 200 and 64 pM respectively. Plant growth regulators like 2,4,5-trichlorophenoxyacetate and 2.4-dichlorophenoxyacetate inhibit oxidative phosphorylation at a concentration of IO-4M. These mitochondria undergo swelling in presence of both KCI and carbon tetrachloride. The swelling induced by KCI is reversed by the addition of succinate but not by the addition of ATP, whereas the swelling: induced by carbon tetrachloride is reversed by ATP and not by the addition of succinate. ATP supported calcium uptake in these mitochondria takes place at lower calcium chloride concentrations.

Item Type:	Article
Uncontrolled Keywords:	cotyledons Phaseolus vulgaris mitochondria
Subjects:	600 Technology > 08 Food technology > 22 Legumes-Pulses
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	22 Dec 2016 07:40
Last Modified:	22 Dec 2016 07:40
URI:	http://ir.cftri.res.in/id/eprint/5103

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