Mohammad Kunhi, A. A. and Singh, R. (1981) Standardisation of assay procedure and some properties of ribonuclease from Aspergillus candidus. Folia Microbiologica, 26 (4). pp. 328-333.
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Abstract
Screening of several fungal cultures resulted in the selection of an isolate of Aspergillus candidus
which produced a considerable amount of RNA-degrading enzyme in both surface and submerged methods
of cultivation. The conditions for the assay of the RNAase were standardized at pH 4.5, 55 ~ and using
0.25 ~o yeast RNA as substrate. The enzyme was stable at pit 5.2. EDTA was found to activate the enzyme
slightly. At temperatures 50-- 60 ~ there was considerable loss in enzyme activity which was traced to the
presence of a contaminating protease which presumably degraded the RNAase optimally at this temperature.
The protease could be preferentially inactivated at or above 75 ~ The crude enzyme, in addition to RNAaso
was found to possess DNAase, nonspecific phosphodiesterase and 3'- and 5'-phosphomonoesterase activities.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Aspergillus candidus, Ribonuclease enzymes, RNAase |
| Subjects: | 500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 03 Fungi 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes |
| Divisions: | Fermentation Technology and Bioengineering |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 05 Mar 2018 08:51 |
| Last Modified: | 05 Mar 2018 08:51 |
| URI: | http://ir.cftri.res.in/id/eprint/5477 |
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