Strategies for large scale inoculum development for solid state fermentation system conidiospores of Trichoderma harzianum.

The suitabllity 01 disc fermenter for efficient production of conidiospores by Trichoderma harzianum is
limited to a workmg capacity of 0.6 1 agar medium due to large decrease in spore production per cm* of the
culture surface area with further increase m Ihc capacity. In contrast, Zymoris, a large scale solid state fermenter
designed at ORSTOM, France and USC of men sohd support for absorbing nutrients offer many advantages
for production of conidmsporcs m quantity sufflclem enough to inoculate pilot and larger fermenters. Five
times higher producuon of comdiosporcs in Zymotis, as compared to the agar methum in flask, constitutes a
success in the development of large scale inoculum.