Studies on groundnut proteins. VI. Isolation, characterisation and hydrogen ion titration of conarachin 11.

A method is described for isolating conarachin II in a homogeneous form by the
techniques of DEAE-cellulose chromatography, polyacrylamide gel electrophoresis
and sedimentation velocity. The protein contains 0.72% carbohydrate
and no phosphate. Hydrogen ion titration curve indicated that the sidechain
carboxyl, imidazole and €-amino groups titrated with normal pKInt values and
their number agreed with the analytical values obtained from amino acid analysis.
However, tyrosine phenolic groups had abnormal pKint of 10.5.