Immobilization of alpha-amylase, glucoamylase and glucose isomerase on CNBr activated sepharose-6MB.

Katwa, L. C. and Raghavendra Rao, M. R. (1983) Immobilization of alpha-amylase, glucoamylase and glucose isomerase on CNBr activated sepharose-6MB. Biotechnology Letters, 5 (3). 191-196, 11 ref..

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Abstract

2 enzymes (glucoamylase from Rhizopus sp. and glucose isomerase from Streptomyces fradiae) or 3 enzymes (the above 2 and alpha-amylase from Bacillus subtilis) were coupled to CNBr-activated Sepharose-6MB and tested for the production of high fructose syrup (HFS) from starch. Some of the parameters such as the pH and temp. activity relationships of the immobilized enzyme systems are described. A 5% soluble starch solution was converted by the enzyme system into a mixture of glucose and fructose within 6 h at 60 degree C.

Item Type:	Article
Uncontrolled Keywords:	IMMOBILIZED-ENZYMES; fructose high syrups, immobilized enzyme systems for; SUGAR-SYRUPS; FRUCTOSE-; immobilized enzyme systems for fructose high syrups
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 07 Enzyme Biochemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	12 Dec 2016 10:29
Last Modified:	12 Dec 2016 10:29
URI:	http://ir.cftri.res.in/id/eprint/6738

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