Studies on Haematococcus pluvialis for improved production of astaxanthin by mutagenesis.

Usha, Tripathi and Venkateshwaran, G. and Sarada, R. and Ravishankar, G. A. (2001) Studies on Haematococcus pluvialis for improved production of astaxanthin by mutagenesis. World Journal of Microbiology and Biotechnology, 17 (2). 143-148, 26 ref..

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Abstract

Culltures of Haematococcus pluvialis were exposed to mutagens, such as UV and EMS (ethyl methanesulphonate), in order to isolate mutants with enhanced astaxanthin productivities. Results showed that the survival rate decreased with increasing UV exposure time and increasing EMS concn. Mutants were screened further using inhibitors of the carotenoid biosynthetic pathway, viz. diphenylamine (15-90muM), nicotine (160-320muM) and compactin (1.5-3.0M). The mutants thus obtained showed enhanced (2.2-3.2-fold) astaxanthin accumulation and also exhibited higher lycopene cyclase activity.

Item Type:	Article
Uncontrolled Keywords:	ALGAE-; CAROTENOIDS-; FERMENTATION-TECHNOLOGY; GENETIC-TECHNIQUES; INHIBITION-; ASTAXANTHIN-; HAEMATOCOCCUS-PLUVIALIS; MUTAGENESIS-
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae 600 Technology > 08 Food technology > 32 Antioxidants
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	19 Jul 2011 04:08
Last Modified:	28 Dec 2011 09:59
URI:	http://ir.cftri.res.in/id/eprint/7643

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