Temperature variations and amyloglucosidase levels at different bed depths in a solid state fermentation system.
Ghildyal, N. P. and Ramakrishna, M. and Lonsane, B. K. and Karanth, N. G. and Krishnaiah, M. M. (1993) Temperature variations and amyloglucosidase levels at different bed depths in a solid state fermentation system. Chemical Engineering Journal, 51 (2). B17-B23, 20 ref..
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Abstract
Studies on moist wheat bran medium, inoculated with Aspergillus niger CFTRI 1105, indicated steep gradients in temp. and enzyme [amyloglucosidase (glucan 1,4-alpha-glucosidase)] levels at different depths in deep bed rectangular fermenters with different loads. An increase of about 2.5C over a bed depth of 40 mm in a fermenter with a load of 49.7 kg m-2 was due to metabolic heat generation in the initial fermentation period. This resulted in a doubled fermentation time to attain enzyme levels comparable with those in a fermenter with a load of 17.8 kg m-2. Temp. variations were about 10-18C for a bed depth of 80 mm and the max. enzyme levels were 23.4% lower with a load of 49.7 kg m-2 compared with those for a load of 17.8 kg m-2. Metabolic heat and enzyme biosynthesis functions were significantly affected by an increase in bed depth. Max. temp. variations recorded in the fermenter with a load of 126.1 kg m-2 were 19.5C at 12 h and 21.2C at 60 h for 80 and 160 mm bed depths, respectively. Consequently, max. enzyme levels were lower by 81-86% and required a 2.5-fold increase in fermentation time compared with a fermenter with a load of 17.8 kg m-2. Results indicate that temp. gradients play a key role in the biosynthesis of enzymes in a solid state fermentation system involving deep beds.
Item Type: | Article |
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Uncontrolled Keywords: | AMYLASES-; ASPERGILLUS-; BIOTECHNOLOGY-; ENZYMES-; FERMENTATION-; FERMENTATION-TECHNOLOGY; FUNGI-; GLUCAN-1,4-alpha-GLUCOSIDASES; NIGER- |
Subjects: | 600 Technology > 05 Chemical engineering > 04 Fermentation Technology |
Divisions: | Fermentation Technology and Bioengineering |
Depositing User: | Food Sci. & Technol. Information Services |
Date Deposited: | 17 Jun 2011 07:31 |
Last Modified: | 28 Dec 2011 10:01 |
URI: | http://ir.cftri.res.in/id/eprint/7938 |
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