Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations

The present article deals with the studies on the
eVect of media ingredients, such as carbon, nitrogen, inorganic
phosphates, surfactants, and metal salts, on phytase
enzyme production by Aspergillus niger CFR 335 in submerged
(SmF) and solid-state fermentations (SSF). The
results obtained showed a 1.5-fold higher enzyme yield in
the presence of sucrose in both SmF and SSF, while peptone
was found to be a favorable nitrogen source for SmF.
Sodium dihydrogen phosphate (NaH2PO4) favored 34%
higher enzyme yield than the control, which was followed
by 19% higher activity in potassium dihydrogen phosphate
(KH2PO4) in SSF at 0.015% w/v. The addition of Tween-
20 in SmF showed a maximum yield of 12.6 U/mL while,
SDS suppressed the growth of the fungus. None of the surfactants
favored the enzyme yield in SSF. Calcium chloride
(CaCl2) was extensively eYcient in stimulating more than
55% higher phytase production in SmF at 0.01% v/v. In
SSF, none of the metal salts stimulated phytase production.