Studies on Microbial Production of Lipoxygenase Inhibitor

Chidananda, C. (2008) Studies on Microbial Production of Lipoxygenase Inhibitor. PhD thesis, University of Mysore.

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Abstract

The thesis reports isolation of several fungal cultures from forest soil and screening of the metabolites for their ability to produce inhibitors against lipoxygenase. Of about 74 isolates, one isolate CFTRI-A-24 found to produce consistently high yields of potent inhibitor, was characterized as Penicillium frequentans. The LOX inhibitor was purified using chromatographic techniques and was identified as “Sclerotiorin”. The inhibition kinetics, mode and mechanism of LOX inhibition, its comparative evaluation to standard compounds, in vitro and in vivo (animal) models evaluation as antioxidant additive and other various biological properties of the inhibitors such as antioxidant, antimicrobial, aldose reductase inhibition, platelet aggregation inhibition, antimutagenic and anticancer property are reported. A medium was designed with optimum physical and nutritional parameters for the efficient production of sclerotiorin with better yields in a lab scale process. Effective recovery of the inhibitor in an economical method was also designed. An effort was made to evaluate sclerotiorin for the possible application as an antioxidant additive in commercial oil sample.

Item Type:	Thesis (PhD)
Uncontrolled Keywords:	lipoxygenase fungal cultures Enzyme inhibitors microbial production
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	07 May 2009 04:40
Last Modified:	28 Dec 2011 10:07
URI:	http://ir.cftri.res.in/id/eprint/8922

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