Aqueous Two Phase Extraction for Purification and Concentration of Biomolecules from Pineapple (Ananas comosus L. Merryl)

Thesis mainly covers the separation, purification and concentration of
biomolecules by aqueous two phase extraction and membrane processes.
Standardization of process parameters to obtain optimal separation during
aqueous two phase extraction and membrane processes is carried out. A
practical approach for simultaneous fractionation and purification of mixture of
enzymes (bromelain and polyphenol oxidase) from pineapple (Ananas
comosus L. Merryl) by aqueous two phase extraction is developed. Major
emphasis is given to develop alternative/newer approaches for the
concentration of biomolecules and liquid foods. Performance evaluation of
direct osmosis process for the concentration of biomolecules (bromelain
enzyme from pineapple waste) and liquid foods (pineapple juice) is carried out
in detail. Modeling of mass and heat transfer in osmotic membrane distillation
process is studied in detail. The basic study of mathematical modeling of the
osmotic membrane distillation/direct osmosis enhances the understanding of
the process. Also, contribution of concentration and temperature polarization
effects during osmotic membrane distillation process is discussed. Integrated
approaches for the purification and concentration of biomolecules are studied
in detail. The proposed protocols will be helpful in developing effective and
efficient processes for the purification and/or concentration of enzymes,
proteins and liquid foods. The major aspects covered in the thesis are the
development of improved/newer processes for the purification and
concentration of biomolecules and liquid foods.
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