Enterotoxigenic Genes in Food Isolates of Staphylococci

Rekha, S. (2010) Enterotoxigenic Genes in Food Isolates of Staphylococci. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	Staphylococcal food poisoning is of major concern in public health programs worldwide. Increased food safety awareness among consumers offers new challenge to control the spoilage and pathogenic organisms in food. The detection of pathogenic organisms in food is of great importance to ensure well being of human kind. World Health Organization also emphasizes on more systemic and aggressive approach to significantly reduce the risk of microbiological food borne diseases. Efficient and rapid detection tools in combination with proper HACCP programmer can help in reducing the risk of food-borne diseases. In present investigation the 6 isolates of Staphylococci from food samples were characterized biochemically. Biochemical tests include gram staining, Catalase test, Phosphatase test, Nitrate test, Urease test, Sugar fermentation test, DNase test, antibiotic sensitivity test etc. Among 6 isolates, two were Staphylococcus epidermidis, and one of each was Staphylococcus haemolyticus, Staphylococcus saprophyticus, Staphylococcus hominis and Staphylococcus xylosus. Isolates were subjected to PCR with accessory gene regulator and sarA primers to confirm their molecular identification. The presence of agr and sarA encoding elements provides a fair indication of the toxigenic potential in Staphylococci. As the food isolates were tested for the presence of ent genes, gave positive result. The presence of regulator genes indicates that the isolates are probably virulent. The quick detection by agr and sarA genes in food samples may help in reducing the incidence of staphylococcal food poisoning.
Uncontrolled Keywords:	Staphylococcal food poisoning; food borne diseases; Staphylococci
Subjects:	600 Technology > 08 Food technology > 09 Food Microbiology
Divisions:	Human Resource Development
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	09 Jul 2010 10:03
Last Modified:	28 Dec 2011 10:15
URI:	http://ir.cftri.res.in/id/eprint/9500

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