Parimalan, R. and Giridhar, P. and Gururaj, H. B. and Ravishankar, G. A. (2009) Micropropagation of Bixa orellana using phytohormones and triacontanol. Biologia Plantarum, 53 (2). pp. 347-350.
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Abstract
An efficient micropropagation protocol for annatto (Bixa orellana L.) was achieved using nodal shoot tip explants.
Shoot buds were obtained on the Murashige and Skoog (MS) medium supplemented with various concentrations and
combinations of indole-3-acetic acid (IAA), N6-benzyladenine (BA) and triacontanol (TRIA). Maximum of 213 shoot
buds along with 18 primary shoots were produced on MS medium containing 0.05 μM IAA, 8.87 μM BA, and 11.2 μM
TRIA. The primary shoots elongated best on MS medium containing 6.66 μM BA and 2.45 μM indole-3-butyric acid
(IBA). The regenerated shoots rooted best on MS medium supplemented with 4.9 μM IBA. The in vitro rooted plantlets
were hardened and establishment rate under field conditions was 70 to 80 %.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | annatto, callus, ex vitro transfer, multiple shoots, organogenesis |
| Subjects: | 500 Natural Sciences and Mathematics > 10 Plants > 06 Trees And Shrubs 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture |
| Divisions: | Plant Cell Biotechnology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 12 Nov 2010 05:38 |
| Last Modified: | 09 Apr 2018 11:19 |
| URI: | http://ir.cftri.res.in/id/eprint/9770 |
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