Purification, Identification, and Characterization of Methylcobalamin from Spirulina platensis.

Kumudha, Anantharajappa and Selva Kumar, Sagaya and Thakur, M. S. and Ravishankar, G. A. and Sarada, R. (2010) Purification, Identification, and Characterization of Methylcobalamin from Spirulina platensis. Journal of Agricultural and Food Chemistry, 58. pp. 9925-9930.

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Abstract

The present study reports methylcobalamin in Spirulina platensis using high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), microbiological assay, chemiluminescence assay, liquid chromatography-mass spectrometry (LC-MS), and tandem mass spectrometry (MS/MS). Extraction of vitamin B12 from S. platensis was carried out without using cyanide. Partial purification was achieved using Amberlite XAD-2 followed by elution with 80% (v/v) methanol. Activated charcoal facilitated removal of impurities in S. platensis extract and in further purification of vitamin B12. The purified fraction was identified to contain methylcobalamin as analyzed by HPLC and TLC. Authenticity of methylcobalamin was further confirmed by LC-MS and MS/MS. Quantitation of methylcobalamin in a test sample of S. platensis biomass was performed using microbiological assay and chemiluminescence assay and was found to be 38.5 ( 2 and 35.7 ( 2 μg/100 g of dry biomass, respectively.

Item Type:	Article
Uncontrolled Keywords:	Spirulina platensis; methylcobalamin; HPLC; chemiluminescence; LC-MS; MS/MS
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae 600 Technology > 08 Food technology > 16 Nutritive value > 04 Vitamins
Divisions:	Fermentation Technology and Bioengineering Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	21 Jul 2011 04:49
Last Modified:	30 May 2012 11:53
URI:	http://ir.cftri.res.in/id/eprint/10301

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