Endogenous profiles of indoleamines: serotonin and melatonin in different tissues of Coffea canephora P ex Fr. as analyzed by HPLC and LC-MS-ESI. fermentation: parameter optimization and application for N-acetyl chitooligosaccharides preparation from chitin.

Ramakrishna, Akula and Giridhar, P. and Udaya Sankar, K. and Ravishankar, G. A. (2012) Endogenous profiles of indoleamines: serotonin and melatonin in different tissues of Coffea canephora P ex Fr. as analyzed by HPLC and LC-MS-ESI. fermentation: parameter optimization and application for N-acetyl chitooligosaccharides preparation from chitin. Acta Physiologiae Plantarum, 34. 393–-396.

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Abstract

Endogenous indoleamine profiles in various ex vitro and in vitro tissues of commercially important Coffea canephora were analyzed by using a high performance liquid chromatography and further confirmed with electrospray ionization mass spectrometry. High content of serotonin (SER) (98.54 ± 5 lg/g) and melatonin (MEL) (115.25 ± 6 lg/g) were found in freshly harvested seeds of C. canephora followed by zygotic embryo (65.25 ± 4 and 96.54 ± 5 lg/g freshweight) and endosperm(34.08 ± 2 and 51.08 ± 4 lg/g fresh weight) of ripened fruits. Similarly endogenous pools of SER and MEL were moderate in in vitro tissues of C. canephora, i.e. callus (25.85 ± 2 and 75.74 ± 4), somatic embryos (31.88 ± 2 and 19.30 ±2 lg/g fresh weight) and in vitro regenerated plant stalk (15.78 ± 1 and 38.25 ± 3 lg/g fresh weight), respectively. In view of significant levels of both SER and MEL in various tissues and beans of Coffea, further investigations on their physiological role needs to be investigated.

Item Type:	Article
Uncontrolled Keywords:	Indoleamines Somatic embryos Tissue culture Zygotic embryos
Subjects:	500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture 600 Technology > 07 Beverage Technology > 04 Coffee
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	05 Aug 2011 11:40
Last Modified:	14 Feb 2012 09:47
URI:	http://ir.cftri.res.in/id/eprint/10420

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