Precursor Biotransformation in Immobilized Placental Tissues of Capsicum frutescens Mill.:* II. Influence of Feeding Intermediates of the Capsaicinoid Pathway in Combination with L -Valine on Capsaicin and Dihydrocapsaicin Accumulation.

Sudhakar Johnson, T. and Ravishankar, G. A. (1998) Precursor Biotransformation in Immobilized Placental Tissues of Capsicum frutescens Mill.:* II. Influence of Feeding Intermediates of the Capsaicinoid Pathway in Combination with L -Valine on Capsaicin and Dihydrocapsaicin Accumulation. Journal of Plant Physiology, 153. 240-243 . ISSN 0176-1617

PDF Journal of Plant Physiology 1998 153 240.pdf - Published Version Restricted to Registered users only Download (492kB)

Abstract

Increased yields of capsaicin and dihydrocapsaicin, major pungent principles of chilli pepper fruit, were obtained when immobilized placental tissues of Capsicum frutescens were treated with intermediate metabolites of the capsaicinoid pathway, i.e. I-phenylalanine, t-cinnamic acid, p-coumaric acid, caffeic acid, ferulic acid and vanillylamine in combination with I-valine. Although vanillylamine and I-valine are the nearest precursors to capsaicin, their addition to immobilized placenta did not significandy affect the accumulation of capsaicin. Maximum capsaicin and dihydrocapsaicin accumulation was achieved on p-coumaric acid + I-valine treatment. Maximum percentage of bioconversion was observed in culture treated with t-cinnamic acid + I-valine. In nature, the ratio of the principle components - capsaicin and dihydrocapsaicin - is about 2: 1. In immobilized placental tissues fed with precursors, the ratio of capsaicin and dihydrocapsaicin varied between 2.2: 1 to 3.7: 1 compared with the ratio of 2: 1 present in natural chilli pepper fruit. A productivity of 3.072 mg capsaicin/gDW/day was obtained by treating with p-coumaric acid + I-valine, w~ was 514 % higher than that of chilli pepper fruit. These results successfully demonstrate the use of placental tissue, the site of synthesis of capsaicin, to enhance yields of metabolites in vitro using a combined precursor treatment.

Item Type:	Article
Uncontrolled Keywords:	Capsaicin, Capsicum frutescens, immobilized plant cells, precursor biotransformation, phenylpropanoid compounds.
Subjects:	500 Natural Sciences and Mathematics > 10 Plants > 01 Plant Cell 600 Technology > 08 Food technology > 30 Spices/Condiments
Divisions:	Planning and Monitoring Cell
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Feb 2012 09:44
Last Modified:	06 Jul 2015 07:25
URI:	http://ir.cftri.res.in/id/eprint/10639

Actions (login required)

View Item