![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Agro-Industrial Residues and Starch For Growth and Co-Production of Polyhydroxyalkanoate Copolymer and alpha-Amylase By Bacillus Sp. Cfr-67
Shamala, T. R. and Vijayendra, S. V. N. and Joshi, G. J. (2012) Agro-Industrial Residues and Starch For Growth and Co-Production of Polyhydroxyalkanoate Copolymer and alpha-Amylase By Bacillus Sp. Cfr-67. Brazilian Journal of Microbiology. pp. 1094-1102.
|
PDF
Brazilian Journal of Microbiology (2012) 1094-1102.pdf - Published Version Download (211kB) | Preview |
Abstract
Polyhydroxyalkanoates (PHA) and α-amylase (-1,4 glucan-4-glucanohydrolase, E.C. 3.2.1.1) were coproduced by Bacillus sp. CFR-67 using unhydrolysed corn starch as a substrate. Bacterial growth and polymer production were enhanced with the supplementation of hydrolysates of wheat bran (WBH) or rice bran (RBH) individually or in combination (5-20 g L-1, based on weight of soluble substrates-SS). In batch cultivation, a mixture of WBH and RBH (1:1, 10 g L-1 of SS) along with ammonium acetate (1.75 g L-1) and corn starch (30 g L-1) produced maximum quantity of biomass (10 g L-1) and PHA (5.9 g L-1). The polymer thus produced was a copolymer of polyhydroxybutyrate-co-hydroxyvalerate of 95:5 to 90:10 mol%. Presence of WBH and corn starch (10-50 g L-1) in the medium enhanced fermentative yield of -amylase (2-40 U mL-1 min-1). The enzyme was active in a wide range of pH (4-9) and temperature (40-60oC). This is the first report on simultaneous production of copolymer of bacterial PHA and -amylase from unhydrolysed corn starch and agro-industrial residues as substrates.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | alpha-amylase, Bacillus sp., Corn starch, Polyhydroxyalkanoate, Rice bran hydrolysate, Wheat bran hydrolysate. |
| Subjects: | 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry 600 Technology > 08 Food technology > 16 Nutritive value > 07 Waste utilization |
| Divisions: | Food Microbiology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 06 Dec 2012 05:51 |
| Last Modified: | 06 Dec 2012 05:51 |
| URI: | http://ir.cftri.res.in/id/eprint/11064 |
Actions (login required)
 |
View Item |