PHO4 transcription factor regulates triacylglycerol metabolism under low-phosphate conditions in Saccharomyces cerevisiae

Kamlesh Kumar, Yadav and Neelima, Singh and Ram, Rajasekharan (2015) PHO4 transcription factor regulates triacylglycerol metabolism under low-phosphate conditions in Saccharomyces cerevisiae. Molecular Microbiology, 98 (3). pp. 456-472.

PDF Molecular Microbiology Volume 98, Issue 3, October 2015, Pages 456–472.pdf - Published Version Restricted to Registered users only Download (11MB)

Abstract

In Saccharomyces cerevisiae, PHM8 encodes a phosphatase that catalyses the dephosphorylation of lysophosphatidic acids to monoacylglycerol and nucleotide monophosphate to nucleoside and releases free phosphate. In this report, we investigated the role of PHM8 in triacylglycerol metabolism and its transcriptional regulation by a phosphate responsive transcription factor Pho4p under lowphosphate conditions. We found that the wild-type (BY4741) cells accumulate triacylglycerol and the expression of PHM8 was high under low-phosphate conditions. Overexpression of PHM8 in the wildtype, phm8Δ and quadruple phosphatase mutant (pah1Δdpp1Δlpp1Δapp1Δ) caused an increase in the triacylglycerol levels. However, the introduction of the PHM8 deletion into the quadruple phosphatase mutant resulted in a reduction in triacylglycerol levels and LPA phosphatase activity. The transcriptional activator Pho4p binds to the PHM8 promoter under low-phosphate conditions, activating PHM8 expression, which leads to the formation of monoacylglycerol from LPA. The synthesized monoacylglycerol is acylated to diacylglycerol by Dga1p, which is further acylated to triacylglycerol by the same enzyme.

Item Type:	Article
Uncontrolled Keywords:	Saccharomyces cerevisiae, triacylglycerol metabolism, yeast
Subjects:	600 Technology > 01 Medical sciences > 12 Metabolism 600 Technology > 08 Food technology > 29 Microbiological food > 04 Yeast
Divisions:	Lipid Science and Traditional Foods
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	17 Feb 2016 08:08
Last Modified:	17 Feb 2016 08:08
URI:	http://ir.cftri.res.in/id/eprint/12107

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