Recombinant endo-mannanase (ManB-1601) production using agro-industrial residues: Development of economical medium and application in oil extraction from copra

Gaurav Singh, Kaira and Deepesh, Panwar and Mukesh, Kapoor (2016) Recombinant endo-mannanase (ManB-1601) production using agro-industrial residues: Development of economical medium and application in oil extraction from copra. Bioresource Technology, 209. pp. 220-227.

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Abstract

Expression of pRSETA manb-1601 construct in Hi-Control Escherichia coli BL21 (DE3) cells improved recombinant endo-mannanase (ManB-1601) production by 2.73-fold (1821 ± 100 U/ml). A low-cost, agro-industrial residue supplemented industrial medium for enhanced and economical production of ManB-1601 was developed in two mutual phases. Phase-I revealed the potential of various pre- (induction time: 5 h, induction mode: lactose 0.5 mM) and post-induction [peptone supplementation: 0.94% (w/v), glycerol 0.123% (v/v)] parameters for enhanced production of ManB-1601 and resulted in 4.61- fold (8406 ± 400 U/ml) and 2.53-fold (3.30 g/l) higher ManB-1601 and biomass production, respectively. Under phase-II, economization of phase-I medium was carried out by reducing/replacing costly ingredients with solubilized-defatted flax seed meal (S-DFSM), which resulted in 3.25-fold (5926 U/ml) higher ManB-1601 production. Industrial potential of ManB-1601 was shown in oil extraction from copra as enzyme treatment led to cracks, peeling, fracturing and smoothening of copra, which facilitated higher (18.75%) oil yield.

Item Type:	Article
Uncontrolled Keywords:	Endo-mannanase Process optimization Industrial medium Flax seed meal Copra oil extraction
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 07 Waste utilization 600 Technology > 08 Food technology > 22 Legumes-Pulses > 06 Coconut
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	25 Aug 2016 08:26
Last Modified:	25 Aug 2016 08:26
URI:	http://ir.cftri.res.in/id/eprint/12256

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