Optimization of culture conditions for bacterial cellulose production from Gluconacetobacter hansenii UAC09

Usha Rani, M. and Anu Appaiah, K. A. (2011) Optimization of culture conditions for bacterial cellulose production from Gluconacetobacter hansenii UAC09. Annals of Microbiology, 61. pp. 781-787.

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Abstract

Fermentation conditions for the production of bacterial cellulose (BC) by a newly isolated Gluconacetobacter hansenii UAC09 were optimized. The effect of various carbon and nitrogen sources on production of BC was determined. The addition of ethanol (1.5%) and acetic acid (1.0%) enhanced BC production by more than three times compared to standard Hestrin and Schramm medium. The strain was able to produce maximum BC (7.40 g l−1) with 4% glucose, 8% corn steep liquor (CSL) at 27±1°C and a pH of 5.5 under stationary conditions. The BC films produced under various conditions were tested for their structural similarity using Fourier transform-infra red analysis and were found to be similar to each other irrespective of medium composition. Scanning electron microscopy images of BC revealed a porous structure with extensively interwoven microfibrils.

Item Type:	Article
Uncontrolled Keywords:	Bacterial cellulose . Fermentation . Optimization . Gluconacetobacter hansenii
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 27 Polymer Chemistry 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Dec 2016 05:58
Last Modified:	08 Dec 2016 05:58
URI:	http://ir.cftri.res.in/id/eprint/12576

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