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Fluorescent competitive aptasensor for detection of aflatoxin B1.
Monali, Mukherjee and Praveena, Bhatt and Manonmani, H. K. (2017) Fluorescent competitive aptasensor for detection of aflatoxin B1. Journal of Molecular Recognition, 30 (12). pp. 1-6.
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Abstract
Aflatoxin B1 (AFB1) is one of the most commonly found mycotoxins in food commodities, particularly cereals, oilseeds, spices and tree nuts. In the past decade, aptamers have come into limelight and emerged as a new biosensing element replacing antibodies in various detection formats. Herein we report a faster, more sensitive, high throughput method for the detection of AFB1 using AFB1‐specific aptamers. The assay format was based on a competitive reaction of the fluorescent tagged aptamer specific to AFB1 with the aflatoxin conjugate. Under optimal conditions, a linear range of detection (50 ng to 50 pg) was achieved with a limit of detection (LOD) of 10 pg/mL in the buffer system. Results of inter‐ and intra‐assay revealed that the assay was repeatable with standard deviation in acceptable range. The assay was also validated in food samples such as dried red chilies, groundnut and whole pepper with recovery in the range of 92 to 102% at 10 ng/mL and 100 pg/mL levels. The aptasensor assay was also compared with standard analytical method of HPLC and was found to be more sensitive. This detection technique has the potential to be developed into a biosensor platform for AFB1 detection.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Aflatoxin B1, aptasensor, competitive binding, fluorescence, food samples |
| Subjects: | 600 Technology > 08 Food technology > 01 Analysis 600 Technology > 08 Food technology > 10 Food Microorganisms |
| Divisions: | Fermentation Technology and Bioengineering Food Protectants and Infestation Control |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 18 Jan 2018 07:00 |
| Last Modified: | 18 Jan 2018 07:00 |
| URI: | http://ir.cftri.res.in/id/eprint/13307 |
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