Improvement of vitamin B12 status with Spirulina supplementation in Wistar rats validated through functional and circulatory markers.

Madhubalaji, C. K. and Rashmi, V. and Chauhan, V. S. and Shylaja, M. Dharmesh and Sarada, R. (2020) Improvement of vitamin B12 status with Spirulina supplementation in Wistar rats validated through functional and circulatory markers. Journal of Food Biochemistry. ISSN 1745-4514

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Abstract

Spirulina evaluated as a source of vitamin B12 through the modulation of vitamin B12 deficiency mediated physiological and biochemical changes in experimental animals. The B12 deficient male weanling Wistar rats were fed with Spirulina‐supplemented diet for 10 weeks. An increase in urinary methylmalonic acid (22.70 ± 4.08 µmol/ moles of creatinine) and plasma homocysteine (16.55 ± 0.48 µmol/L) levels in the B12 deficient group was observed, while these were equal to control in the Spirulina fed group (8.71 ± 0.48 µmol/mol of creatinine and 6.88 ± 1.18 µmol/L, respectively). The vitamin B12 levels in serum (874.27 ± 89.69), plasma (615.53 ± 26.5 pg/ml), kidney (10.19 ± 1.066 ng/g), and liver tissues (6.37 ± 0.62 ng/g) in the Spirulina fed group were similar to control. Severe atrophic changes in the testes and altered tissue ar‐ chitecture in lung and spleen as seen in the B12 deficient group were normalized in the Spirulina fed group. The study validates that Spirulina can improve the vitamin B12 status. Practical applications The present study showed that the supplementation of Spirulina in the diet of vitamin B12 deficient rats leads to the normalization of vitamin B12 deficiency‐induced circu‐ latory and functional biomarkers along with biochemical and histological changes. Vegetarian sources for vitamin B12 are limited and the results presented here provide scientific validation for the use of Spirulina as a potential vegetarian source of bio‐ available vitamin B12

Item Type:	Article
Uncontrolled Keywords:	B12 deficiency, histology, homocysteine, methylmalonic acid, Spirulina, vitamin B12
Subjects:	500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 01 Algae 600 Technology > 08 Food technology > 16 Nutritive value > 04 Vitamins
Divisions:	Dept. of Biochemistry Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	01 Oct 2020 04:59
Last Modified:	01 Oct 2020 04:59
URI:	http://ir.cftri.res.in/id/eprint/14483

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