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Sequence characterization and N-glycoproteomics of secretory immunoglobulin A from donkey milk.
Gnanesh Kumar, B. S. and Apoorva, Rawal (2020) Sequence characterization and N-glycoproteomics of secretory immunoglobulin A from donkey milk. International Journal of Biological Macromolecules (155). pp. 605-613. ISSN 0141-8130
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Abstract
Secretory immunoglobulin A (sIgA) is the major antibody present in the human milk where it confers passive immunity to neonates. Other than human, non-ruminants such as equine, swine etc., also possess sIgA in milk but detailed characterization is limited. In the present study, we characterized sIgA from donkey milk for amino acid sequence and N-glycosylation through LC-MS/MS analysis. The complete amino acid sequence of alpha chain constant region (CH) was elucidated. The sequence analysis of variable regions (VH and VL) and light chain constant region (CL) showed several amino acid substitutions indicating the presence of diverse immunoglobulin repertoire. Glycoproteomic analysis of secretory component revealed bi-antennary complex and hybrid types with differential core fucosylation at site N83LT, only complex glycans at N135GT, N423GT and N530LT with mainly NeuAc whereas N291QT harbors high mannose glycans. Heavy chain possesses majorly bi-antennary complex with differential core fucosylation at sites N139AS and N338VS, in which N338VS shows partial occupancy. Joining chain harbors only complex type at N72IS, with core fucosylation and terminal NeuGc to some extent. N-glycan repertoire in part is similar to human sIgA. This comprehensive analysis of sequence and glycan pattern of donkey milk sIgA would be beneficial for its potential applications.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Donkey milk Immunoglobulin A N-glycosylation |
| Subjects: | 500 Natural Sciences and Mathematics > 11 Animals 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins 600 Technology > 08 Food technology > 27 Dairy products |
| Divisions: | Dept. of Biochemistry |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 27 Nov 2020 09:11 |
| Last Modified: | 27 Nov 2020 09:11 |
| URI: | http://ir.cftri.res.in/id/eprint/14661 |
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