Phenolic Extract of Seagrass, Halophila ovalis Activates Intrinsic Pathway of Apoptosis in Human Breast Cancer (MCF-7) Cells.

Harshita, Sharma and Nimish Mol, Stephen and Sowmyashree, G. and Deepika, U. R. and Yogendra Prasad, K. and Arpitha, H. S. and Hithamani, G. and Ganesan, P. (2021) Phenolic Extract of Seagrass, Halophila ovalis Activates Intrinsic Pathway of Apoptosis in Human Breast Cancer (MCF-7) Cells. Nutrition and Cancer, 73 (2). pp. 307-317.

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Abstract

The marine ecosystem is considered as a treasure of numerous novel biologically active molecules. We investigated the anticancer potential of the phenolic extract of Halophila ovalis in breast cancer (MCF-7) cells and characterized the possible underlying molecular mechanism. The phenolic extract (5 ml) of H. ovalis effectively inhibited the growth of MCF-7 cells. The results of DAPI staining indicated that this phenolic extract potently induces apoptosis in MCF-7 cells which was observed by increased chromatin condensation in the treated cells. An increased expression of the active fragments of an executioner caspase, caspase 3 in phenolic extract-treated MCF-7 cells further confirms this apoptosis induction. In consequence, the loss of mitochondrial membrane potential was noticed in treated cells. The protein expression analyzes show decreased expression of the anti-apoptotic protein, Bcl-2, and DNA repair enzyme, PARP in treated cells indicating the probable molecular targets of apoptosis. Further, the phenolic extract of H. ovalis blocked the antioxidant defense system in MCF-7 cells by down-regulating the protein expression of a major transcription factor, Nrf-2 and regulatory antioxidant enzymes, SOD-2 and HO-1. These results show the presence of chemopreventive compound(s) in the phenolic extract, which offers a platform for future studies to identify the active principles.

Item Type:	Article
Uncontrolled Keywords:	anticancer potential, phenolic extract, Halophila ovalis, breast cancer
Subjects:	600 Technology > 01 Medical sciences > 09 Human Physiology 600 Technology > 08 Food technology > 28 Meat, Fish & Poultry > Fish
Divisions:	Molecular Nutrition
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	07 Jan 2021 05:37
Last Modified:	29 Nov 2021 10:58
URI:	http://ir.cftri.res.in/id/eprint/14759

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