Exogenous Serotonin and Melatonin Regulate Dietary Isoflavones Profoundly through Ethylene Biosynthesis in Soybean [Glycine max (L.) Merr.]

Gyanendra, Kumar and Kirti, R. Saad and Bijesh, Puthusseri and Monisha, Arya and Nandini, P. Shetty and Giridhar, P. (2021) Exogenous Serotonin and Melatonin Regulate Dietary Isoflavones Profoundly through Ethylene Biosynthesis in Soybean [Glycine max (L.) Merr.]. Journal of Agricultural and Food Chemistry, 69. pp. 1888-1899. ISSN 0021-8561

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Abstract

Serotonin and melatonin are important signaling and stress mitigating molecules. However, their role and molecular mechanism in the accumulation of isoflavones are not clearly defined. To elucidate their functions, serotonin and melatonin were applied to in vitro cultures of soybean at different concentrations and analyzed to assess the accumulation of isoflavone content followed by transcript levels of biosynthesis genes at different time intervals. Increased total phenolics, total flavonoids, and different forms of isoflavone content were observed in the treatments. Expression levels of critical genes in isoflavone, ethylene, jasmonic acid, abscisic acid, and melatonin biosynthesis and related transcription factor were quantified. A correlation was observed between the expression of ethylene biosynthesis genes (S-adenosylmethionine synthase and 1-aminocyclopropane-1-carboxylate oxidase) and isoflavone biosynthesis genes (chalcone synthase, chalcone reductase, and isof lavone synthase). We hypothesize that, under serotonin and melatonin treatments, ethylene biosynthesis may play a role in the increase/decrease in isoflavone content in soybean culture.

Item Type:	Article
Uncontrolled Keywords:	chalcone synthase, ethylene biosynthesis, isoflavone synthase, melatonin role, serotonin role
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 04 Biosynthesis 600 Technology > 08 Food technology > 22 Legumes-Pulses > 05 Soya bean
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	15 Jul 2021 10:48
Last Modified:	15 Jul 2021 10:48
URI:	http://ir.cftri.res.in/id/eprint/14891

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