Profiling of bioactives and in vitro evaluation of antioxidant and antidiabetic property of polyphenols of marine algae Padina tetrastromatica.

Naveen, J. and Revathy, Baskaran and Baskaran, V. (2021) Profiling of bioactives and in vitro evaluation of antioxidant and antidiabetic property of polyphenols of marine algae Padina tetrastromatica. Algal Research, 55. p. 102250.

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Abstract

This study aimed to characterize and quantify the phenolics, hydrocolloids, lipids and carotenoids and to study antioxidant and antidiabetic property of phenolics of marine brown algae Padina tetrastromatica. Extraction using 1% acidic methanol yielded 3.6 mg/g total polyphenol, 2.86 mg/g flavonoid and 21.8 μg/g tannin. Ultra high performance liquid chromatography-high resolution mass spectrometry/mass spectrometry (UPLC-HRMS/MS) analysis resulted in free phenolics: danshensu, quercetin derivative, luteolin, genistein, and hydroxyl-ferulic acid derivative; bound phenolics: luteolin, acacetin derivative, dioxinodehydroeckol derivative, and rosmarinic acid. The radical scavenging activities of the phenolic extracts were 10.83 ± 2.81 and 58.85 ± 2.28 μg/mL by the 2,2′ - azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) methods, respectively. The total antioxidant activity of seaweed phenolics was 29.13 ± 2.31 μg of ascorbic acid equivalent, and the reductive capability was 32.39 ± 3.11 μg of quercetin equivalent. In addition, the extract exhibited a half maximal inhibitory concentration (IC50) of α-amylase (47.2 ± 2.9 μg) and α-glucosidase (28.8 ± 2.3 μg) inhibitory activities. Fatty acids methyl-palmitate and methyl-palmitoleate were present in neutral-, glyco-, and phospholipid fractions of total lipids (0.82%). The seaweed is rich in bioactives fucoxanthin (96.66%), polysaccharides (mg/g dry wt.) laminarin (14.78), fucoidan (1.38) and alginate (70.16) respectively. Based on the nutraceutical density, there will be potential food and pharma applications for P. tetrastromatica.

Item Type:	Article
Uncontrolled Keywords:	Marine seaweed Padina tetrastromatica Nutraceuticals Ultra-high performance liquid chromatography-high-resolution mass spectrometry Gas chromatography Phenolic compounds
Subjects:	500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 01 Algae 600 Technology > 08 Food technology > 18 Processed foods > 04 Functional foods
Divisions:	Dept. of Biochemistry Fruit and Vegetable Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	23 Nov 2021 08:56
Last Modified:	23 Nov 2021 08:56
URI:	http://ir.cftri.res.in/id/eprint/15065

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