Comparative assessment of antioxidant and immunomodulatory properties of skimmed milk protein hydrolysates and their incorporation in beverage mix.

Pavan Kumar, P. and Sachin, M. Eligar and Prakruthi, M. and Jyothi Lakshmi, A. (2022) Comparative assessment of antioxidant and immunomodulatory properties of skimmed milk protein hydrolysates and their incorporation in beverage mix. Journal of the Science of Food and Agriculture, 102. pp. 6414-6422. ISSN 0022-5142

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Abstract

BACKGROUND: Milk-derived protein hydrolysates have generated a great deal of interest recently due to their numerous ben�eficial health effects. However, there are few comparative studies on protein hydrolysates from different dairy species, their production, characterization, and bioactivity. In the present study, skimmed milk from both major and minor dairy species was hydrolyzed with alcalase, and its protein profiles were studied using tricine polyacrylamide gel electrophoresis and reverse phase-high protein liquid chromatography. The antioxidant and in vitro immunostimulatory properties were determined. RESULTS: Iron chelation activity was highest in hydrolysates of whey (25.00 ± 0.32 mmol L−1 ), casein (25.14 ± 0.34 mmol L−1 ), colostrum (24.52 ± 0.28 mmol L−1 ), and skimmed cattle milk (24.21 ± 0.26 mmol L−1 ). ⊍,⊍-Diphenyl-⊎-picrylhydrazyl scaveng�ing and 2,20 -azobis(2-amidino-propane) dihydrochloride activity was lowest in skimmed donkey milk protein hydrolysates (MPHs) (IC50: 5.37 ± 0.05 mg mL−1 and 151.59 ± 2.1 mg mL−1 ). Production of nitric oxide and phagocytosis activity in RAW 264.7 (murine macrophage cell line) was significantly higher among whey and buffalo skimmed milk protein hydrolysate�treated groups as compared with the untreated group. The incorporation of whey protein hydrolysate and skimmed buffalo milk protein hydrolysate were sensorially acceptable at 10% level in beverage mix. CONCLUSION: This study comparatively evaluates the antioxidative and immunomodulatory properties of different skimmed MPHs and their potential applications as ingredients in pediatric, geriatric, and other health-promoting foods. © 2022 Society of Chemical Industry

Item Type:	Article
Uncontrolled Keywords:	Milk proteins; protein hydrolysates; antioxidant activity; enzymatic hydrolysis; RAW 264.7 macrophages; immunomodul�ation; beverage mix
Subjects:	600 Technology > 07 Beverage Technology 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins 600 Technology > 08 Food technology > 27 Dairy products
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	23 May 2023 08:58
Last Modified:	23 May 2023 08:58
URI:	http://ir.cftri.res.in/id/eprint/16438

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