Prevalence of macrolide–lincosamide–streptogramin resistant lactic acid bacteria isolated from food samples.

Ashwini, M. and Mousumi, Ray and Sumana, K. and Prakash, M. Halami (2023) Prevalence of macrolide–lincosamide–streptogramin resistant lactic acid bacteria isolated from food samples. Journal of Food Science and Technology, 60 (2). pp. 630-642. ISSN 0022-1155

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Abstract

Lactic acid bacteria (LAB) being a reservoir of antibiotic resistance genes, tend to disseminate antibiotic resistance that possibly pose a threat to human and animal health. Therefore, the study focuses on the prevalence of macrolide-lincosamide-streptogramin- (MLS) resistance among LAB isolated from various food samples. Diverse phenotypic and genotypic MLS resistance were determined among the LAB species (n=146) isolated from fermented food products (n=6) and intestine of food-producing ani�mals (n=4). Double disc, triple disc difusion and stand�ard minimum inhibitory concentration (MIC) tests were evaluated for phenotypic MLS resistance. Specifc primers for MLS resistance genes were used for the evaluation of genotypic MLS resistance and gene expressions using total RNA of each isolate at diferent antibiotic concentrations. The isolates identifed are Levilactobacillus brevis (n=1), Enterococcus hirae (n=1), Limosilactobacillus fermen�tum (n=2), Pediococcus acidilactici (n=3), Enterococ‑ cus faecalis (n=1). The MIC tests along with induction studies displayed cMLSb, L phenotype, M phenotype, KH phenotype, I phenotype resistance among MLS antibiotics. Genotypic evaluation tests revealed the presence of ermB, mefA/E, msrA/B and msrC genes. Also, gene expression studies displayed increased level of gene expression to the twofold increased antibiotic concentrations. In the view of global health concern, this study identifed that food samples and food-producing animals represent source of antibiotic resistant LAB that can disseminate resistance through food chain. This suggests the implementation of awareness in the use of antibiotics as growth promoters and judicious use of antibiotics in veterinary sectors in order to prevent the spread of antibiotic resistance

Item Type:	Article
Uncontrolled Keywords:	Antibiotic resistance · Lactic acid bacteria · Resistance genes · Erythromycin · Clindamycin · Gene expression
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology 600 Technology > 08 Food technology > 18 Processed foods
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	26 May 2023 04:37
Last Modified:	26 May 2023 04:37
URI:	http://ir.cftri.res.in/id/eprint/16475

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