Downstream Processing Of Polygalacturonase and Xanthophylls From Aspergillus Carbonarius Using Membrane Technology

Nakkeeran, E. (2005) Downstream Processing Of Polygalacturonase and Xanthophylls From Aspergillus Carbonarius Using Membrane Technology. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	<p align="justify">Extensive review of research reports published in the area of membrane processing of enzymes revealed that the ultrafiltration membranes were best suited for concentration and purification of enzymes. The molecular weight of enzyme ranges from 10-100 kDa and the researchers generally used a 10 kDa UF membrane independent of the molecular weight of the enzyme. However, a proper selection of membrane process specific for each process is desirable. In this investigation, a set UF membranes varying in MWCO (10-100 kDa) were screened considering the reported the molecular weight of polygalacturonase (~42 kDa) produced by the mutant strain of Aspergillus carbonarius. A MF (150 nm) membrane was used as a prefilter for reducing the polysaccharide content in the culture filtrate. This fungi also produces xanthophylls (pigment) having an average molecular weight of 574 Da. The pigment is extracted using organic solvents and partial desolventization was attempted using a hydrophilic NF membrane.</p>
Uncontrolled Keywords:	Polygalacturonase Xanthophylls Membrane Technology Aspergillus carbonarius
Subjects:	600 Technology > 08 Food technology > 07 Food Engineering 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi 600 Technology > 05 Chemical engineering > 03 Enzyme Biotechnology and Engineering
Divisions:	Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	12 Sep 2005
Last Modified:	28 Dec 2011 09:25
URI:	http://ir.cftri.res.in/id/eprint/172

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