Fractionation and purification of the phycobiliproteins from Spirulina platensis.

Patil, Ganapathi and Chethana, S. and Madhusudhan, M. C. and Raghavarao, K. S. M. S. (2008) Fractionation and purification of the phycobiliproteins from Spirulina platensis. Bioresource Technology, 99 (15). pp. 7393-6. ISSN 0960-8524

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Abstract

C-Phycocyanin and allophycocyanin of Spirulina platensis are fractionated and purified using a non-chromatographic method namely, aqueous two phase extraction for the first time. Optimized process parameters of aqueous two phase extraction (PEG 4000/potassium phosphate of tie line length 18.64% with a phase volume ratio 1.45) resulted in pure C-phycocyanin and allophycocyanin with a purity of 3.23 and 0.74, respectively, in a single extraction. Multiple extractions (two) improved the purity of C-phycocyanin from 3.23 to 4.02. Integration of aqueous two phase extraction with membrane process not only facilitated the separation of phase forming components from the products and also increased the purity of allophycocyanin from 0.74 to 1.5.

Item Type:	Article
Uncontrolled Keywords:	C-Phycocyanin; Allophycocyanin; Aqueous two phase extraction; Fractionation; Purification
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
Divisions:	Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Jun 2009 06:28
Last Modified:	21 Dec 2016 13:00
URI:	http://ir.cftri.res.in/id/eprint/1810

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