Semi-commercial scale fermentation of prawn waste by Exiguobacterium sp. GM010 for bioactive pigment production: Safety and efficacy assessment in Wistar rats.

Krishna Prashanth, Ramesh Mekala and Muthukumar, S. P. and Mohan, A. Dhale (2024) Semi-commercial scale fermentation of prawn waste by Exiguobacterium sp. GM010 for bioactive pigment production: Safety and efficacy assessment in Wistar rats. Food and Bioproducts Processing (2024), 147. pp. 189-198.

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Abstract

Pigment producing Exiguobacterium sp. GM010 demonstrates utilization of prawn carapace hydrolysate. Accordingly, our investigation was focused to produce cost effective bioactive pigment in 15 L semi-commercial scale. Further acute and subacute toxicities were evaluated as per OECD guidelines for application as nutraceutical. The biomass and pigment extract yield were 28.76 ± 1.78 and 0.82 ± 0.04 g/L, respectively, at 1.5 L of aeration. The UV–visible spectrum of pigment showed absorption peaks at 357, 466, and 491 nm indicating carotenoid like pigment. This was correlated with colour distribution in yellowish-orange region of CIE Lab spectrum. The qualitative and quantitative characteristics of pigment were similar between lab-scale and bioreactor production. The acute toxic dose (2000 mg/kg bw) exhibited the toxicity as determined by haematological analysis. While subacute toxic dose (250, 500, 1000 mg/kg bw) demonstrated no adverse effects on parameters such as food intake, body weight gain, vital organ weight, haematological and histopathological examination. Serum biochemistry results showed significant increase in HDL and decrease in SGPT values in treated groups compared to control. The results revealed that, Exiguobacterium sp. GM010 has efficiently utilised prawn waste to produce bioactive pigment. The dose of Exiguobacterium sp. GM010 pigment lower than 1000 mg/kg bw can be regarded as safe.

Item Type:	Article
Uncontrolled Keywords:	Exiguobacterium sp Upscale Valorisation Prawn carapace Microbial pigments Toxicity
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 07 Waste utilization 600 Technology > 08 Food technology > 28 Meat, Fish & Poultry > Fish
Divisions:	Dept. of Biochemistry Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Nov 2024 08:35
Last Modified:	08 Nov 2024 08:35
URI:	http://ir.cftri.res.in/id/eprint/18435

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