Glutamic acid-induced shoot differentiation in tobacco callus tissues and changes in nicotine content and in activities of aminotransferases, ornithine transcarbamylase, and arginase.

Ravishankar, G. A. and Mehta, A. R. (1991) Glutamic acid-induced shoot differentiation in tobacco callus tissues and changes in nicotine content and in activities of aminotransferases, ornithine transcarbamylase, and arginase. Biochemistry International, 23 (4). pp. 679-87. ISSN 0158-5231

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Abstract

Floral bud calluses of Nicotiana tabacum L. var. Anand-2 produced multiple shoots on Murashige and Skoog's (MS) medium supplemented with 2 mg/l indole-3-acetic acid (IAA), 0.4 mg/l kinetin (Kn), and L-glutamic acid (2.5 mM). However cultures of calluses on MS medium containing only the IAA and Kn did not differentiate shoots. Specific activities of phenylalanine aminotransferase, tyrosine aminotransferase, and ornithine aminotransferase were over 7-, 5- and 5-fold higher, respectively, in the glutamic acid-treated calluses than in the nonshoot-forming ones on the day of shoot emergence. Activities of ornithine transcarbamylase and arginase decreased after the onset of the differentiation process. Implications of the enzyme activities in the differentiation process and in nicotine biogenesis are discussed.

Item Type:	Article
Uncontrolled Keywords:	nicotine, shoot differentiation, aminotransferase, tobacco callus
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	12 Nov 2021 10:30
Last Modified:	12 Nov 2021 10:30
URI:	http://ir.cftri.res.in/id/eprint/2333

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