Production of cellulases and D-xylanase by some selected fungal isolates.

Shamala, T. R. and Sreekantiah, K. R. (1986) Production of cellulases and D-xylanase by some selected fungal isolates. Enzyme and Microbial Technology, 8 (3). pp. 178-182.

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Abstract

Cellulase [see 1,4-(1,3;1,4)-~-D-glucan 4-glucanohydrolase, EC 3.2.1.4], (J-D-glucosidase (~-D-glucoside glucanohydrolase, EC 3.2.1.21) and D-xylanase (],4-~-D-xylan xylanohydrolase, EC 3.2.1.8) production by Aspergillus ustus, Sporotrichum pulverulentum, Trichoderma sp. (a), Trichoderma sp. (b) and Botrytis sp. in solid state fermentation on different compounded media containing wheat bran {WB), rice straw (RS) and minerals was studied. Toyama's mineral solution mixed with RS was found to be a better substrate for cellulase and D-xylanase while with WB it induced higher ~-Oglucosidase production. A ratio of substrate to mineral solution (w/v) of 1:4 or 1:5 supported high D-xylanase and cellulase production whereas a ratio of 1:2 gave the highest [J-D-glucosidase activity. Among the fungal isolates, Aspergillus ustus gave the highest [J-D-glucosidase activity of 60 U g -1 WB and the highest D-xylanase activity of 740 U g-1 was obtained with RS. A mixture of seven parts of RS and three of WB, mixed with 40 parts of Toyama's mineral solution yieMed 6 U filter paper activity, 40 U ~-D-glucosidase, 12 U carboxymethylcellulase and 650 U D-xylanase g-1 substrate.

Item Type:	Article
Uncontrolled Keywords:	Cellulase; D-xylanase; O-D-glucosidase; rice straw; wheat bran; solid state fermentation; nutrient medium volumes; fungi
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Jun 2011 10:47
Last Modified:	28 Dec 2011 09:49
URI:	http://ir.cftri.res.in/id/eprint/5071

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