Stabilization of cetyltrimethyl ammonium bromide permeabilized yeast whole cell lactase.

Cetyltrimethylammonium bromide-permeabilized cells ofK. fragilis
loose ~-galactosidase activity due to leaking of the enzyme into
the medium. This leakage of the enzyme can be prevented by storing
the permeabilized cells either in buffer containing 50,% glycerol
or by treating the permeabilized cells with 0.2,% glutaraldehyde at
4~ for 10 min. In repeated batch hydrolysis of lactose in milk,
glutaraldehyde treated cells could be repeatedly used very efficiently.