Enzymatic acidolysis in hexane to produce n-3 or n-6 FA-enriched structured lipids from coconut oil: optimization of reactions by response surface methodology.

Reena, Rao and Balaraman, Manohar and Sambaiah, K. and Lokesh, B. R. (2002) Enzymatic acidolysis in hexane to produce n-3 or n-6 FA-enriched structured lipids from coconut oil: optimization of reactions by response surface methodology. Journal of the American Oil Chemists' Society, 79 (9). pp. 885-890.

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Abstract

Response surface methodology is a statistical design that helps determine optimal conditions for an enzyme-catalysed reaction using the min. number of experiments. This methodology was adapted for modifying coconut oil triacylglycerols (TAG) using lipase-catalysed acidolysis in hexane to incorporate n-3 or n-6 PUFA. Free fatty acids (FFA) obtained after hydrolysis of cod liver oil and safflower oil were used as acyl donors. Immobilized lipase (Lipozyme IM60 from Rhizomucor miehei) was used to catalyse the reaction. Reaction conditions - substrate molar ratio, incubation time and temp. - were optimized. Experimental data were fitted to a response function based on the central composite rotatable design. Optimal conditions generated from models indicated that max. incorporation of n-3 PUFA occurred at a 1:4 molar ratio of TAG-FFA when incubation was carried out for 34 h at 54C. Similarly, max. incorporation of n-6 fatty acids was predicted at a 1:3 molar ratio of TAG/FFA when incubated for 48.5 h at 39C. Experiments conducted at optimized conditions predicted by the equation obtained from response surface methodology yielded structured lipids with 13.65 and 45.5% of n-3 and n-6 fatty acids, respectively. These values agreed well with those predicted by the model. Reactions scaled up to 100 g levels in batch reactors had incorporation levels of n-3 and n-6 fatty acids agreeing closely with those observed when reactions were carried out at lab scale (100 mg). It is concluded that response surface methodology is a useful tool in predicting conditions for incorporating desired levels of specific fatty acids during synthsis of structured lipids.

Item Type:	Article
Uncontrolled Keywords:	ANALYTICAL-TECHNIQUES; LIPASES-; LIPIDS-; OILS-VEGETABLE; COCONUT-OILS; RESPONSE-SURFACE-METHODOLOGY; STRUCTURED-LIPIDS
Subjects:	600 Technology > 08 Food technology > 19 Lipids-oils/fats 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions:	Food Engineering Lipid Science and Traditional Foods Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	01 Jul 2009 06:10
Last Modified:	28 Dec 2011 09:53
URI:	http://ir.cftri.res.in/id/eprint/6359

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