Development of cellulase-free xylanase-producing anaerobic consortia for the use of lignocellulosic wastes.

Rani, S. and Nand, K. (1996) Development of cellulase-free xylanase-producing anaerobic consortia for the use of lignocellulosic wastes. Enzyme and Microbial Technology, 18 (1). 23-28, 24 ref..

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Abstract

Consortia of anaerobic bacteria producing xylan degrading enzymes were developed from samples collected from various ecosystems. Consortia developed included a thermophilic consortium (KK-10) producing cellulase-free xylanase (410 nkat ml-1) at 65-85C, pH 7-9, and mesophilic consortia (PC-4, CC-2 and BD-2) exhibiting xylanase activities of 580, 275 and 300 nkat ml-1, respectively, but also producing cellulases in the culture medium. Temp. and pH influenced the yield and pattern of xylanase and cellulase production markedly, with the optimum at 72 h of growth and pH 6.7. Max. xylanase activity was recorded for all consortia at 37C except PC-4 and KK-10 consortia, which showed their highest activity at 55 and 65C, respectively. BD-2 consortium when cultivated at 55C exhibited a several-fold increase in beta-glucosidase activity as compared with other consortia. It also had a broad pH range of 5-9 for growth and xylanase production. A pH of 6-7 was optimum for other consortia. Max. xylanase activity of 592 nkat ml-1 was detected with PC-4 consortium. The xylanase of KK-10 could find industrial applications at higher temp., whereas neutral xylanases may be used in the food-processing industries. [From En summ.]

Item Type:	Article
Uncontrolled Keywords:	BACTERIA-; ENZYMES-; GLYCOSIDASES-; MICROORGANISMS-; XYLAN-DEGRADING-ENZYMES
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	26 Nov 2008 10:56
Last Modified:	03 Oct 2018 09:09
URI:	http://ir.cftri.res.in/id/eprint/7860

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