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Aldose Reductase Of Oryza Sativa: Purification And Characterisation

Jeety Ram, T (2005) Aldose Reductase Of Oryza Sativa: Purification And Characterisation. [Student Project Report]

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This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: <p align="justify">Aldose reductase (EC; alditol:NADPH oxidoreductase) belong to the well-conserved aldo-keto reductase super family of enzymes in plants and animals. They are monomeric, cytosolic proteins that catalyze the NADPH dependent reduction of aldoses and other aldehydes to their corresponding alcohols. In plants ARD is induced in vegetative tissues in response to drought and stress. Since it has been strongly implicated in the development of the chronic complications of diabetes mellitus in animals, much effort has been devoted to understanding the structure and mechanism of this enzyme. The enzyme aldose reductase was partially purified and characterized from Rice bran. The reductase, a monomer had an average molecular weight of 34.5 kDa as determined by gel filtration on Biosep-2000 and SDS-PAGE. The enzyme was NADPH specific and reduced a broad range of substrates including aldoses, aliphatic aldehydes and aromatic aldehydes. Maximum activity was observed at pH-6.9 in sodium phosphate buffer using DL-glyceraldehyde as substrate. Even though the main physiological role of aldose reductase is not clear, it plays a major role as a key enzyme in the polyol pathway.</p>
Uncontrolled Keywords: enzymes aldose reductase rice oryza sativa
Subjects: 600 Technology > 08 Food technology > 21 Cereals > 01 Rice
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 23 Jun 2005
Last Modified: 28 Dec 2011 09:24
URI: http://ir.cftri.res.in/id/eprint/84

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