Production of Galactooligosaccharides by using β-Galactosidase from Lactic Acid Bacteria

Kruthika, K. (2009) Production of Galactooligosaccharides by using β-Galactosidase from Lactic Acid Bacteria. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	There is considerable interest from the pharmaceutical industries as well as food industry to take advantage of the beneficial properties of galactooligosaccharides and its products.This study reports that the intracellular β-galactosidase produced by Lactobacillus spp. (Reddy et al., 2007) a kanjika isolate from CFTRI grown on reconstituted MRS broth was capable of performing transgalactosylation reaction with lactose as substrate. Under the optimized conditions the GOS yield is 34.5% which is quite significant. An HPLC and ESI/MS result indicates that the GOS mixture contains G3, G4, and G5. There is convincing evidence from the immobisation experiments that the monosaccharides and lactose can be removed from the reaction mixture by immobilized Lactobacillus beads. Further studies require improvement of enzmye yield, production of high content GOS and scale-up the GOS production.
Uncontrolled Keywords:	β-galactosidase production Lactobacillus species Prebiotic Galacto-oligosaccharides
Subjects:	600 Technology > 05 Chemical engineering > 04 Fermentation Technology 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	04 Jun 2009 09:41
Last Modified:	28 Dec 2011 10:08
URI:	http://ir.cftri.res.in/id/eprint/9002

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