A response surface methodological study on prediction of glucosylation yields of thiamin using immobilized [beta]-glucosidase

Ponrasu, Thangavel and Manohar, Balaraman and Divakar, S. (2009) A response surface methodological study on prediction of glucosylation yields of thiamin using immobilized [beta]-glucosidase. Process Biochemistry, 44 (2). pp. 251-255. ISSN 1359-5113

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Abstract

Response surface methodology was used to predict the glucosylation yields of thiamin using immobilized [beta]-glucosidase. A Central Composite Rotatable Design (CCRD) of 32 experiments with immobilized [beta]-glucosidase, thiamin, incubation period, buffer concentration and pH, as five independent variables was employed at five levels. A second-order polynomial equation was developed, the regression coefficient values of which exhibited a R2 value of 0.74. Contour plots explained the glucosylation behaviour of the enzyme through a reversal in glucosylation at a cross-over point corresponding to 60% (w/w d-glucose) immobilized [beta]-glucosidase and 0.12mM buffer concentration at pH 6. The highest conversion yield of 58% obtained experimentally compared well with the predicted yield of 52% under optimum conditions of 40% immobilized [beta]-glucosidase, 0.55mmol thiamin, 96h incubation period and 0.16mM buffer concentration at pH 7. Validation experiments carried out at certain random predictive conditions also showed good correspondence between experimental and predictive yields.

Item Type:	Article
Uncontrolled Keywords:	CCRD Glucosylation Immobilized [beta]-glucosidase RSM Reversal in glucosylation Thiamin glucoside
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 04 Vitamins 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions:	Fermentation Technology and Bioengineering Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	18 Jun 2009 04:17
Last Modified:	28 Dec 2011 10:09
URI:	http://ir.cftri.res.in/id/eprint/9060

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