An automated flow injection analysis system for on-line monitoring of glucose and L-lactate during lactic acid fermentation in a recycle bioreactor

Kumar, M. A. and Thakur, M. S. and Senthuran, A. and Senthuran, V. and Karanth, N. G. (2001) An automated flow injection analysis system for on-line monitoring of glucose and L-lactate during lactic acid fermentation in a recycle bioreactor. World Journal of Microbiology and Biotechnology, 17. pp. 23-29.

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Abstract

The study concerns on-line sequential analysis of glucose and L-lactate during lactic acid fermentation using a ¯ow injection analysis (FIA) system. Enzyme electrodes containing immobilized glucose oxidase and L-lactate oxidase were used with an amperometric detection system. A 12-bit data acquisition card with 16 analog input channels and 8 digital output channels was used. The software for data acquisition was developed using Visual C++, and was devised for sampling every hour for sequential analyses of lactate and glucose. The detection range was found to be 2±100 g l)1 for glucose and 1±60 g l)1 for L-lactate using the biosensors. This FIA system was used for monitoring glucose utilization and L-lactate production by immobilized cells of Lactobacillus casei subsp. rhamnosus during a lactic acid fermentation process in a recycle batch reactor. After 13 h of fermentation, complete sugar utilization and maximal L-lactate production was observed. A good agreement was observed between analysis data obtained using the biosensors and data from standard analyses of reducing sugar and L-lactate. The biosensors exhibited excellent stability during continuous operation for at least 45 days.

Item Type:	Article
Uncontrolled Keywords:	Biosensor, flow injection analysis, glucose, lactic acid fermentation, L-lactate, on-line monitoring
Subjects:	600 Technology > 05 Chemical engineering > 04 Fermentation Technology 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology
Divisions:	Central Instrumentation Facility Services Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	30 Jun 2009 09:06
Last Modified:	14 Dec 2016 10:45
URI:	http://ir.cftri.res.in/id/eprint/9131

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