Probiotic lactic acid bacterium from kanjika as a potential source of vitamin B12: evidence from LC-MS, immunological and microbiological techniques

Arenahalli, N. Madhu and Prashant, Giribhattanavar and Mandayam Singara, Narayan and Prapulla, S. G. (2010) Probiotic lactic acid bacterium from kanjika as a potential source of vitamin B12: evidence from LC-MS, immunological and microbiological techniques. Biotechnology Letters.

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Abstract

Vitamin B12 was produced by probiotic Lactobacillus plantarum in submerged fermentation (96 h) with successive anaerobic and aerobic phases of 48 h each to give 13 ng vitamin B12/g dry biomass. Sodium cyanide-mediated cell lysis, followed by benzyl alcohol/chloroform/water extraction, improved the release of intracellular vitamin B12 for analysis. The presence of the K+ adduct of cyanocobalamin (m/z of 1394) was established using electron spray ionization–mass spectra; growth of a mutant of Escherichia coli in the presence of cyanocobalamin ascertained its bioavailability.

Item Type:	Article
Uncontrolled Keywords:	Cyanocobalamin E. coli ATCC 11105 Lactic acid bacteria Mass spectra Probiotics
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	25 Feb 2010 11:02
Last Modified:	27 Apr 2012 06:44
URI:	http://ir.cftri.res.in/id/eprint/9341

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