Isolation, Screening of Fungi from Soil and Food Grains and their Antimicrobial Activity

Rohini, H.K. (2010) Isolation, Screening of Fungi from Soil and Food Grains and their Antimicrobial Activity. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	Fungi were isolated from different sources . All isolates were subjected for secondary metabolite production in Antibiotic test broth. Out of 19 isolates most of them shows clear zone of inhibition against some plant pathogenic fungi such as F.graminerum, Rhizopus, T.paradox and also some human pathogenic bacteria such as Staph. aureus, S. typhi, B.cereus and E.coli . Identification of fungal organisms are done only for those organisms which have shown anti microbial activity. The results of anti bacterial and anti fungal activities were compared with the standard. For fungicidal activity Carbendazium, 98% pure was used and for bactericidal activity Streptomycin sulphate was used. The isolates bearing No 8th,9th,18th,22nd,25th,29th,30th,31st showed very good activity against all bacteria ( E. coli, B.cereus, S .typhi, Staph.aureus) . The isolate No 29 against E. coli shows activity almost equal to that of the standard (Streptomycin sulphate). The isolate bearing No 10th,14th,17th,19th, 20th and 28th have shown moderate activity. Least bactericidal activity has been reported from the isolates bearing No 3rd, 6th,13th,and 21st and no activity has been reported from the isolate bearing No 15th. The results indicated that the antifungal activity of fungal extracts bearing No 3rd and 10th were almost comparable to the standard against F. graminerum . Similarly, isolate bearing No 9th and 29th have shown very good activity against Rhizopus species. Also the isolate bearing No 14th ,15th, 20th and 21st have shown almost similar activity to that of the standard CBZ against T. paradox. Among all fungal extracts, No bearing 14th and 21st have shown greater activity than any other extracts against T. paradox, which is more sensitive than other two fungi tested. The above results indicate that it is possible to isolate and identify certain newer fungal secondary metabolites which could act upon to control certain plant pathogens as well as bacterial pathogens. Further studies are needed to elucidate the exact compounds responsible for these biological activities.
Uncontrolled Keywords:	Fungi; antimicrobial activity; secondary metabolites; plant pathogenic fungi; food grains
Subjects:	600 Technology > 08 Food technology > 21 Cereals 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi
Divisions:	Food Protectants and Infestation Control
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	09 Jul 2010 05:19
Last Modified:	28 Dec 2011 10:15
URI:	http://ir.cftri.res.in/id/eprint/9487

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