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Solid state fermentation production of chitin deacetylase by Colletotrichum lindemuthianum ATCC 56676 using different substrates
Suresh, P. V. and Sachindra, N. M. and Bhaskar, N. (2011) Solid state fermentation production of chitin deacetylase by Colletotrichum lindemuthianum ATCC 56676 using different substrates. Journal of Food Science and Technology, 48 (3). pp. 349-356.
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Abstract
Production of extracellular chitin deacetylase by Colletotrichum lindemuthianum ATCC 56676 under solid substrate fermentation was studied. The suitability of shrimp shell chitin waste (SSCW) and commercial wheat bran (CWB) was evaluated for maximal enzyme production. CWB medium (pH 6.4±0.2) supplemented with chitosan favoured maximal chitin deacetylase yield of 460.4±14.7 unit/g initial dry substrate (U/g IDS) at 96 h as compared to maximal yield of 392.0±6.4 U/g IDS at 192 h in SSCW medium (pH 8.7±0.2) at 25 °C incubation temperature and 60% (w/w) initial moisture content of medium. Along with chitin deacetylase, C. lindemuthianum ATCC 56676 produced maximum endo-chitinase (0.28± 0.03 U/g IDS at 144 h) and β-N-acetylhexosaminidase (0.79±0.009 U/g IDS at 192 h) in CWB medium and 0.49± 0.05 U/g IDS of endo-chitinase at 264 h and 0.38±0.04 U/g IDS of β-N-acetylhexosaminidase at 96 h of incubation in SSCW medium. SEM studies indicated the difference in the morphology of mycelia and hyphae of C. lindemuthianum ATCC 56676 when grown on different solid substrates. Production of chitin deacetylase by SSF is being reported for the first time.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Colletotrichum lindemuthianum ATCC 56676 . Chitin deacetylase . Solid state fermentation . Wheat bran . Shrimp shell chitin waste |
| Subjects: | 600 Technology > 05 Chemical engineering > 04 Fermentation Technology 600 Technology > 08 Food technology > 28 Meat, Fish & Poultry |
| Divisions: | Meat Fish and Poultry Technology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 21 Jun 2011 07:26 |
| Last Modified: | 28 Dec 2011 10:24 |
| URI: | http://ir.cftri.res.in/id/eprint/10149 |
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